Coleman & Co. Member Reference
Research peptides arrive as a dry powder (lyophilized) in a sterile vial. Before injecting, you dissolve — reconstitute — the powder using bacteriostatic water. This creates a liquid solution you can measure and inject with an insulin syringe.
Think of it like mixing baby formula: the powder is the active ingredient; the water is the carrier. Getting the math right means getting your dose right.
Sterile water with 0.9% benzyl alcohol. Keeps the solution bacteria-free for up to 28 days.
U-100, 0.5 mL or 1 mL with 30g or 31g needle. Markings in units (100 units = 1 mL).
70% isopropyl alcohol. Wipe every vial top before inserting a needle — every single time.
Use the Peptide Calculator to verify your draw volume. No mental math.
Clean your workspace. Lay out your supplies. Remove caps but keep everything sterile. Work on a clean surface — a fresh paper towel works.
Use a fresh alcohol wipe on the peptide vial top AND the bacteriostatic water vial top. Let them dry for 10 seconds. Do not blow on them — that adds bacteria.
Uncap your insulin syringe. Pull the plunger back to your target volume before inserting into the bac water vial. Insert the needle into the bac water vial. Inject the air (this prevents a vacuum). Then invert the vial and draw your water slowly — no bubbles.
Hold the peptide vial at a 45° angle. Insert the needle and drip the water down the inside wall of the vial — do NOT shoot it directly onto the powder. This prevents foam and protects delicate peptide chains from shear damage.
Gently roll the vial between your palms or swirl in small circles. Never shake or vortex — peptides are fragile amino acid chains and rough handling can destroy them. Wait 30-60 seconds for complete dissolution.
Write on the vial (or on a small label): compound name, reconstitution date, concentration (mg/mL), and your calculated draw volume in units. A Sharpie on glass works. Date matters — reconstituted peptides last 21-28 days refrigerated.
| Typical vial | 10 mg or 12 mg |
| Bac water | 1 mL or 2 mL (per your protocol card) |
| Dosing schedule | Once weekly SubQ injection |
| Starting dose | 2 mg/week, titrate per protocol |
| Storage | Refrigerate at 2–8°C after reconstitution. Use within 28 days. |
| Syringe type | U-100 insulin, 0.5 mL or 1 mL, 30g–31g |
| Typical vial | 5 mg / 5 mg blend or per compounding pharmacy label |
| Bac water | 2 mL (standard for most blends) |
| Dosing schedule | Once or twice daily SubQ, fasted — typically at bedtime and/or upon waking |
| Timing | Inject on an empty stomach. Wait 30+ minutes before eating. This maximizes GH pulse. |
| Storage | Refrigerate at 2–8°C after reconstitution. Use within 21 days. |
| Syringe type | U-100 insulin, 0.3 mL or 0.5 mL, 30g–31g |
Store in the freezer at −20°C. The powder is stable for 12–24 months when kept frozen and dry. Do not open the vial until you're ready to reconstitute — moisture degrades the peptide.
Store in the refrigerator at 2–8°C (36–46°F). Do NOT freeze after reconstitution — ice crystals destroy peptide bonds. Keep the vial capped and protected from light. Most reconstituted peptides last 21–28 days refrigerated.
Use an insulin travel case with a cold pack. Keep vials upright. Never leave reconstituted peptides in a hot car or direct sunlight. Room temperature for a few hours is fine — but get them back into refrigeration as soon as possible.